Composite

Part:BBa_K609005:Design

Designed by: Ke Liu   Group: iGEM11_Harbin_Inst_of_Tech_Food_   (2011-10-02)


human collagen type Ⅲ+ nisI (without promoter)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 514
    Illegal EcoRI site found at 559
    Illegal XbaI site found at 574
    Illegal SpeI site found at 1479
    Illegal PstI site found at 1493
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 559
    Illegal SpeI site found at 515
    Illegal SpeI site found at 1479
    Illegal PstI site found at 529
    Illegal PstI site found at 1493
    Illegal NotI site found at 15
    Illegal NotI site found at 522
    Illegal NotI site found at 565
    Illegal NotI site found at 1486
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 559
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 515
    Illegal EcoRI site found at 559
    Illegal XbaI site found at 574
    Illegal SpeI site found at 1479
    Illegal PstI site found at 1493
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal EcoRI site found at 559
    Illegal XbaI site found at 24
    Illegal XbaI site found at 574
    Illegal SpeI site found at 515
    Illegal SpeI site found at 1479
    Illegal PstI site found at 529
    Illegal PstI site found at 1493
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1106


Design Notes

This part can reconstruct with a vector without any antibiotic gene for a safer use in food.


Source

The collagen gene comes from the α1 chain of human collagen type Ⅲ (ColⅢ) and the nisI is found in Lactococcus lactis.

References