![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K609005:Design
Designed by: Ke Liu Group: iGEM11_Harbin_Inst_of_Tech_Food_ (2011-10-02)
human collagen type Ⅲ+ nisI (without promoter)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 9
Illegal suffix found in sequence at 514
Illegal EcoRI site found at 559
Illegal XbaI site found at 574
Illegal SpeI site found at 1479
Illegal PstI site found at 1493 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 9
Illegal EcoRI site found at 559
Illegal SpeI site found at 515
Illegal SpeI site found at 1479
Illegal PstI site found at 529
Illegal PstI site found at 1493
Illegal NotI site found at 15
Illegal NotI site found at 522
Illegal NotI site found at 565
Illegal NotI site found at 1486 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 9
Illegal EcoRI site found at 559 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 9
Illegal suffix found in sequence at 515
Illegal EcoRI site found at 559
Illegal XbaI site found at 574
Illegal SpeI site found at 1479
Illegal PstI site found at 1493 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 9
Illegal EcoRI site found at 559
Illegal XbaI site found at 24
Illegal XbaI site found at 574
Illegal SpeI site found at 515
Illegal SpeI site found at 1479
Illegal PstI site found at 529
Illegal PstI site found at 1493 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1106
Design Notes
This part can reconstruct with a vector without any antibiotic gene for a safer use in food.
Source
The collagen gene comes from the α1 chain of human collagen type Ⅲ (ColⅢ) and the nisI is found in Lactococcus lactis.